The attraction of chemiluminescence as an analytical tool lies in the simplicity of detection. The essence of chemiluminescence is self-luminescence, which means that chemiluminescence analysis test instruments only need to provide a method to detect optical signals and record results. Self-illuminating detectors require a closed sample chamber and photodetector. The easiest is photo paper or X-ray film, and even a visual detector.
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The simplicity of the chemiluminescence detection method makes its application simple and fully automated. But what about its sensitivity? Chemiluminescence has two inherent advantages:
1. Most samples do not have a "background" signal, as they do not emit light themselves.
2. Chemiluminescence detection is not a proportional test, which is different from fluorescence and absorption or colorimetric tests. Fluorescent groups with small StokesShift are very difficult to detect in fluorescence testing. Fluorescence is difficult to distinguish from the excitation wavelength.
Another problem is that a portion of the stray light will enter the detector, especially if the sample is turbid. 